Is there a way to calibrate the A/F ratio without having the engine on a dyno? If so how do I do it and what do I need?

Sure, what exhaust do you have? Have a bung in welded in your exhaust and use a wideband O2 sensor to monitor. You could use a gage or a handheld monitor. There are a bunch of kits out there.
I have a Holley MPI kit and I can adjust mine with the laptop.

I'm running K/E headers, I'm going to have the tails modified so I'll have them add a bung in the tail pipe.

Do you have EFI or carb? You can install a wideband O2 as jeffswav said to measure the A/F ratio. Here is where you can get one at. http://www.innovatemotorsports.com/products/lm2.php

I'm running a carb.

Just be sure they will not get wet...

Not to hijack the thread but I am also looking to monitor A/F ratio and the product shown looks interesting. Can you just "monitor" the A/F ratio or can you change it? I have an adjustable fuel regulator so I know I can change fuel pressure but if I do not have the correct A/F ratio do I need to have my ECM re-programed...??? (Running a Procharger @ 5lbs on a 454 Mag MPI))

You can check it with the monitor, if it is off have one of the guys here reprogram it for you.
Mine is cool because I can do it myself and I can set target A/F ratios and let the PCM tweak it out.

You can use you fuel press to offset the fuel curve but it won't change the shape of it.

Jeff

Just trying to wrap my head around this......so if I find I have the A/F ratio a little "lean" at say 12 or 13, I can find this out with this kit but would have to send the ECM in to be re-programed? Or at those numbers, (I was told about 11.5 is what I should be running) can I richen it up a little with higher fuel pressure???

Jeffswav, what do you mean by letting the PCM "tweak" it out..? Thanks.